Roseicyclus Medium (DSMZ Medium 1183)

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Roseicyclus Medium
(DSMZ Medium 1183)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 SO 4 .......................................................................................15.0g
MgSO 4 ..........................................................................................2.0g
Malic acid ....................................................................................1.0g
Na acetate .....................................................................................1.0g
Yeast extract .................................................................................1.0g
Peptone ........................................................................................0.5g
KCl ...............................................................................................0.3g
NH 4 Cl ..........................................................................................0.3g
Bicarbonate solution..................................................................5.0mL
Phosphate solution.....................................................................3.0mL
Vitamin solution ........................................................................2.0mL
Trace elements solution.............................................................2.0mL
Calcium chloride solution..........................................................0.5mL
pH 8.3 ± 0.2 at 25°C
Phosphate Solution:
Composition per 10.0mL:
K 2 HPO 4 .........................................................................................1.0g
Preparation of Phosphate Solution:  Add K 2 HPO 4 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Calcium Chloride Solution:
Composition per 10.0mL:
CaC l 2 ·2H 2 O ..................................................................................1.0g
Preparation of Calcium Chloride Solution:  Add CaC l 2 ·2H 2 O
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature.
Bicarbonate Solution :
Composition per 10.0mL:
NaHCO 3 ........................................................................................1.0g
Preparation of Bicarbonate Solution:  Add NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Adjust
pH to 6.5. Filter sterilize.
Vitamin Solution:
Composition per liter:
Nicotinic acid................................................................................0.4g
Thiamine-HCl·2H 2 O.....................................................................0.4g
Biotin .......................................................................................80.0mg
Vitamin B 12 ................................................................................0.5mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Trace Elements Solution:
Composition per liter:
FeSO 4 ·7H 2 O..................................................................................0.3g
ZnSO 4 ·7H 2 O..............................................................................5.0mg
MnCl 2 ·4H 2 O...............................................................................3.0mg
NiCl 2 ·6H 2 O................................................................................3.0mg
Na 2 MoO 4 ·4H 2 O .........................................................................3.0mg
H 3 BO 3 ........................................................................................2.0mg
CuCl 2 ·2H 2 O ...............................................................................2.0mg
CoCl 2 ·6H 2 O ...............................................................................1.0mg
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.0. Filter sterize.
Preparation of Medium: Add components, except phosphate, bi-
carbonate, calcium chloride, trace elements, and vitamin solutions, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Adjust pH to 5.95. Distribute into tubes or flasks. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Aseptically add phosphate, bicarbonate, calcium
chloride, trace elements, and vitamin solutions. Mix thoroughly. Asep-
tically distribute into culture vessels.
Use: For the cultivation of Roseicyclus spp.