Roseinatronobacter Agar (DSMZ Medium 928)

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Roseinatronobacter Agar
(DSMZ Medium 928)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 .......................................................................................25.0g
Na 2 CO 3  .......................................................................................11.0g
NaHCO 3 ........................................................................................4.0g
NaCl..............................................................................................2.5g
Sodium acetate..............................................................................0.8g
Yeast extract..................................................................................0.5g
Peptone..........................................................................................0.5g
KNO 3 ..........................................................................................0.25g
Agar solution..........................................................................500.0mL
pH 10.0 ± 0.2 at 25°C
Agar Solution:
Composition per 500.0mL:
Agar ............................................................................................20.0g
Preparation of Agar Solution:  Add agar to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 55°C.
Preparation of Medium: Add components, except agar solution, to
distilled/deionized water and bring volume to 500.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C. Add
500.0mL sterile warm agar solution. Mix thoroughly. Pour into Petri
dishes or distribute to sterile tubes.
Use: For the cultivation of Roseinatronobacter thiooxidans.