SC Broth without Uracil

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

SC Broth without Uracil

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 ....................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
NaCl..............................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Inositol.......................................................................................2.0mg
KI...............................................................................................1.0mg
H 3 BO 3 ........................................................................................0.5mg
ZnSO 4 ·7H 2 O..............................................................................0.4mg
MnSO 4 ·4H 2 O.............................................................................0.4mg
Thiamine·HCl............................................................................0.4mg
Pyroxidine·HCl..........................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin..................................................................................0.2mg
FeCl 3 ..........................................................................................0.2mg
Na 2 MoO 4 ·4H 2 O.........................................................................0.2mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Folic acid ....................................................................................2.0μg
Biotin..........................................................................................2.0μg
Glucose solution......................................................................50.0mL
Synthetic stock solution...........................................................30.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................40.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Synthetic Stock Solution:
Composition per 300.0mL:
L -Isoleucine.................................................................................5.25g
L -Arginine...................................................................................3.48g
L -Aspartic acid............................................................................2.66g
L -Leucine....................................................................................2.62g
L -Methionine...............................................................................1.49g
L -Threonine.................................................................................1.19g
L -Valine.......................................................................................1.17g
L -Serine.......................................................................................1.05g
L -Lysine ......................................................................................0.91g
L -Phenylalanine ..........................................................................0.83g
L -Tryptophan ..............................................................................0.82g
L -Histidine ..................................................................................0.58g
Myo-inositol ...............................................................................0.36g
L -Tyrosine...................................................................................0.18g
Adenine.....................................................................................0.135g
Preparation of Synthetic Stock Solution: Add components to
distilled/deionized water and bring volume to 300.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except synthetic stock
solution and glucose solution, to distilled/deionized water and bring
volume to 920.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 30.0mL of sterile synthetic stock solution and 50.0mL
of sterile glucose solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation of strains of Saccharomyces cerevisiae that do
not require uracil.