SC Medium (DSMZ Medium 751)

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

SC Medium
(DSMZ Medium 751)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone from soy meal .................................................................8.0g
KH 2 PO 4 .........................................................................................1.5g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Hemin solution.........................................................................15.0mL
Serum albumin solution...........................................................10.0mL
Cysteine solution......................................................................10.0mL
Glucose solution ........................................................................5.0mL
pH 6.6 ± 0.2 at 25°C
Hemin Solution:
Composition per 100.0mL:
Hemin........................................................................................... 0.1g
Preparation of Hemin Solution: Add hemin to 100mL 0.05N
NaOH. Mix thoroughly.
Serum Albumin Solution:
Composition per 10.0mL:
Bovine serum albumin, fraction V............................................... 2.0g
Preparation of Serum Albumin Solution: Add bovine serum al-
bumin, fraction V to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O .....................................................................1.0g
Preparation of Cysteine Solution:  Add  L -cysteine·HCl·H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, serum albumin solution, and cysteine solution, to distilled/deion-
ized water and bring volume to 970.0mL. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°C. Aseptically add 10.0mL sterile cysteine solution,
10.0mL sterile glucose solution, and 10.0mL sterile serum albumin so-
lution. Mix thoroughly. Adjust pH to 6.6. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of Leifsonia xyli subsp. cynodontis.