SC Medium

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

SC Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1021.0mL:

Agar............................................................................................15.0g
Papaic digest of soybean meal......................................................8.0g
Corn meal, solids from infusion ...................................................2.0g
Tween™ 80...................................................................................1.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Hemin chloride solution ..........................................................15.0mL
Bovine serum albumin solution...............................................10.0mL
L -Cysteine solution..................................................................10.0mL
Glucose solution........................................................................1.0mL
pH 6.6 at 25°C
Hemin Chloride Solution:
Composition per 100.0mL:
Hemin chloride .............................................................................0.1g
NaOH (0.05N solution)..........................................................100.0mL
Preparation of Hemin Chloride Solution: Add hemin chloride
to 100.0mL of NaOH solution. Mix thoroughly.
Bovine Serum Albumin Solution:
Composition per 10.0mL:
Bovine serum albumin..................................................................2.0g
Preparation of Bovine Serum Albumin Solution: Add bovine
serum albumin to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
L -Cysteine Solution:
Composition per 10.0mL:
L -Cysteine, free base.....................................................................1.0g
Preparation of  L -Cysteine Solution: Add  L -cysteine to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Glucose Solution:
Composition per 10.0mL:
Glucose.........................................................................................5.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components—except bovine serum
albumin solution,  L -cysteine solution, and glucose solution—to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile bovine se-
rum albumin solution, 10.0mL of sterile  L -cysteine solution, and
1.0mL of sterile glucose solution. Mix thoroughly. Pour into sterile Pe-
tri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of coryneform bacteria that
cause ratoon stunting disease of sugarcane.