SCGYEM Medium

[所属分类:培养基配方] [发布时间:2021-8-11] [发布人:网站管理员2] [阅读次数:] [返回]

SCGYEM Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casein, isoelectric.......................................................................10.0g
Yeast extract................................................................................. 5.0g
Glucose.........................................................................................2.5g
Na 2 HPO 4 ...................................................................................1.325g
KH 2 PO 4 .........................................................................................0.8g
Calf serum, heat inactivated ..................................................100.0mL
Preparation of Medium: Add components, except calf serum, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Autoclave for 30 min at 15 psi pressure–121°C. Cool to 25°C. Asep-
tically add 100.0mL of sterile, heat-inactivated calf serum. Mix
thoroughly. Aseptically distribute into sterile, screw-capped tubes or
flasks.
Use: For the cultivation of Naegleria australiensis and Naegleria
fowleri.