Seawater Lemco Agar

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Lemco Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Seawater, filtered aged...........................................................750.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 7.8. Gently heat and bring to boiling. Boil for 3–5 min. Filter
through Whatman filter paper. Adjust pH to 7.3. Add agar. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes or leave in tubes.
Use:  For the cultivation of Halococcus nondenitrificans.