Seawater Lemco Broth

[所属分类:培养基配方] [发布时间:2021-8-12] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Lemco Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Seawater, filtered aged...........................................................750.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.8. Gently
heat and bring to boiling. Boil for 3–5 min. Filter through Whatman filter
paper. Adjust pH to 7.3. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C.
Use:  For the cultivation of Halococcus nondenitrificans.