Seawater 802 Medium, Half-Strength

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater 802 Medium, Half-Strength

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL
Solution B..............................................................................500.0mL
Solution A:
Composition per 500.0mL:
NaCl............................................................................................27.5g
MgCl 2 ·6H 2 O ...............................................................................5.38g
MgSO 4 ·7H 2 O.............................................................................6.78g
CaCl 2 ·2H 2 O ..................................................................................1.4g
KCl..............................................................................................0.72g
NaHCO 3 ....................................................................................... 0.2g
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly. Filter sterilize.
Solution B:
Composition per liter:
Rye grass cerophyll.......................................................................2.5g
NaH 2 PO 4 ...................................................................................... 0.5g
Preparation of Solution B: Add cerophyll to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to a boil. Boil for 5 min. Filter through Whatman #1 filter paper. Add
0.5g of Na 2 HPO 4 . Bring volume to 1.0L with distilled/deionized water.
Mix thoroughly. Distribute 10.0mL volumes into tubes. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Source: Cerophyll can be obtained from Ward's Natural Science Es-
tablishment, Inc. Dairy Goat Nutrition distributes Grass Media Cul-
ture, which is equivalent. Cereal Leaf Product from Sigma Chemical is
similar to cerophyll.
Preparation of Medium: Aseptically add 500.0mL of sterile solu-
tion A and 500.0mL of sterile solution B. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of Euplotes harpa.