Seawater Nitrosomonas Medium

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Nitrosomonas Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1003.3mL:

HEPES (N-[2-hydroxyethyl]piperazine-N′-2-
ethanesulfonic acid) buffer...............................................4.76g
(NH 4 ) 2 SO 4 ....................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O .............................................................................20.0mg
K 2 HPO 4 ....................................................................................15.0mg
Artificial seawater.........................................................................1.0L
K 2 CO 3 (5% solution).................................................................2.0mL
Trace elements solution.............................................................1.0mL
Phenol Red (0.04% solution).....................................................0.3mL
pH 7.8 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
EDTA..........................................................................................2.06g
FeSO 4 ·7H 2 O................................................................................1.54g
MnCl 2 ·4H 2 O..................................................................................0.2g
Na 2 MoO 4 ·2H 2 O ............................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O............................................................................20.0mg
CoCl 2 ·6H 2 O ...............................................................................2.0mg
HCl, concentrated ....................................................................83.0mL
Preparation of Trace Elements: Add HCl and EDTA to distilled/
deionized water and and bring volume to 900.0mL. Mix thoroughly.
Add remaining components. Mix thoroughly.
Artificial Seawater:
Composition per liter:
NaCl............................................................................................27.5g
MgSO 4 ·7H 2 O..............................................................................6.78g
MgCl 2 ·6H 2 O................................................................................5.38g
CaCl 2 ·2H 2 O...................................................................................1.4g
KCl..............................................................................................0.72g
NaHCO 3 ........................................................................................0.2g
Preparation of Artificial Seawater: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Combine components. Mix thoroughly.
Adjust pH to 7.8. Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C.
Use: For the cultivation of Nitrosomonas cryotolerans.