Seawater Nutrient Agar (SNA) (ATCC Medium 2205)

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Nutrient Agar
(SNA)
(ATCC Medium 2205)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Nutrient agar, 2X....................................................................500.0mL
Synthetic seawater, 2X...........................................................500.0mL
pH 7.3 ± 0.2 at 25°C
Nutrient Agar, 2X
Composition per 500.0mL:
Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Beef extract...................................................................................1.0g
Preparation of Nutrient Agar, 2X: Add components to distilled/
deionized water and bring volume to 500.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C.
Seawater, Synthetic, 2X:
Composition per 500.0mL:
NaCl............................................................................................24.0g
MgSO 4 ·7H 2 O................................................................................7.0g
MgCl 2 ·6H 2 O..................................................................................5.3g
KCl................................................................................................0.7g
CaCl 2 .............................................................................................0.1g
Preparation of Seawater, Synthetic, 2X: Add components to
distilled/deionized water and bring volume to 500.0mL. Mix thorough-
ly. Adjust pH to 7.5. Filter sterilize.
Preparation of Medium: Warm synthetic seawater to 50°C. Asep-
tically combine sterile nutrient agar and sterile synthetic seawater. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the isolation and cultivation of marine bacteria.