Seawater Yeast Extract Peptone Medium

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Yeast Extract Peptone Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................3.0g
Seawater, aged and filtered....................................................750.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 7.8. Gently heat and bring to boiling. Continue boiling for
3–5 min. Filter through Whatman filter paper. Adjust pH to 7.3. Add
agar. Gently heat and bring to boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri
dishes or leave in tubes.
Use:  For the cultivation of Planococcus kocurii.