Seawater Yeast Peptone Agar (DSMZ Medium 243)

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Seawater Yeast Peptone Agar
(DSMZ Medium 243)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................12.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................3.0g
Artificial seawater..................................................................750.0mL
pH 7.3 ± 0.2 at 25°C
Artificial Seawater:
NaCl..........................................................................................28.13g
MgCl 2 ·6H 2 O..................................................................................4.8g
MgSO 4 ·7H 2 O................................................................................3.5g
CaCl 2 ·2H 2 O...................................................................................1.6g
KCl..............................................................................................0.77g
NaHCO 3 ......................................................................................0.11g
Preparation of Artificial Sea Water: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add peptone and yeast extract to
250.0mL distilled/deionized water. Mix thoroughly. Adjust pH to 7.8.
Boil for 5 min. Filter and readjust the pH to 7.3. Add agar. Gently heat
and bring to boiling. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°C–50°C. Add 750.0mL fil-
ter-sterilized seawater that has been heated to 50°C. (Note: Natural sea-
water is stored in the dark for at least 3 weeks to age. If natural seawater
is not available use artificial seawater.) Mix thorougly. Pour into sterile
Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Zobellia uliginosa=Cellu-
lophaga uliginosa=Cytophaga uliginosa, Marinobacterium janna-
schii=Oceanospirillum jannaschii, Vibrio harveyi=Lucibacterium har-
veyi, Halomonas sp., Pseudoalteromonas espejiana=Alteromonas espe-
jiana.