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Selenite Broth (Selenite Broth, Lactose) (Selenite F Enrichment Medium) (Sodium Biselenite Medium) (Sodium Hydrogen Selenite Medium)

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]
Selenite Broth
(Selenite Broth, Lactose)
(Selenite F Enrichment Medium)
(Sodium Biselenite Medium)
(Sodium Hydrogen Selenite Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................10.0g
Pancreatic digest of casein............................................................5.0g
Lactose..........................................................................................4.0g
NaHSeO 3 ·5H 2 O ............................................................................4.0g
pH 7.0 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and a prepared medium from Oxoid Unipath.
Caution: Sodium biselenite is toxic and a potential teratogen and care
must be taken to avoid inhalation of the powdered dye, contact with the
skin, or ingestion, especially in pregnant laboratory workers.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Distribute into sterile tubes in 10.0mL
volumes.
Use: For the isolation and enrichment of Salmonella species from clin-
ical specimens and food products.
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