Selenite Broth Base, Mannitol

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Selenite Broth Base, Mannitol

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 .....................................................................................10.0g
Peptone..........................................................................................5.0g
Mannitol........................................................................................4.0g
NaHSeO 3 ·5H 2 O ............................................................................4.0g
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Caution:  Sodium selenite is toxic and a potential teratogen and care
must be taken to avoid inhalation of the powdered dye, contact with the
skin, or ingestion, especially in pregnant laboratory workers.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat. Do not
autoclave. Distribute into sterile tubes in 10.0mL volumes. Sterilize for
10 min at 0 psi pressure–100°C.
Use: For the isolation and cultivation of Salmonella typhi and Salmo-
nella paratyphi.