Sellers Agar (Sellers Differential Agar)

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Sellers Agar
(Sellers Differential Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1015.0mL:

Pancreatic digest of gelatin.........................................................20.0g
Agar............................................................................................13.5g
D- Mannitol ....................................................................................2.0g
NaCl..............................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.5g
K 2 HPO 4 .........................................................................................1.0g
L- Arginine.....................................................................................1.0g
NaNO 3 ..........................................................................................1.0g
Yeast extract..................................................................................1.0g
NaNO 3 ........................................................................................0.35g
Bromthymol Blue.......................................................................0.04g
Phenol Red.................................................................................8.0mg
Glucose solution......................................................................15.0mL
pH 6.7 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................5.0g
Preparation of Glucose Solution: Add  D -glucose to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into tubes in
10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Al-
low tubes to cool in a slanted position to form a 3-inch slant with a 1.5-
inch butt. Immediately prior to inoculation, aseptically add 0.15mL of
sterile glucose solution to each tube. Let the glucose solution run down
the side of the tube opposite the slant.
Use:  For the cultivation and differentiation of nonfermentative Gram-
negative bacilli, especially Pseudomonas aeruginosa, Herellea vagini-
cola (Acinetobacter calcoaceticus), Mima polymorpha (Acinetobacter
lwoffii), Alcaligenes faecalis, and Bacterium anitratum (Acinetobacter
calcoaceticus).