Sellers Differential HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Sellers Differential HiVeg Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone ..............................................................................20.0g
Agar............................................................................................15.0g
D -Mannitol....................................................................................2.0g
NaCl..............................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.5g
K 2 HPO 4 .........................................................................................1.0g
L -Arginine.....................................................................................1.0g
Sodium nitrate...............................................................................1.0g
Yeast extract..................................................................................1.0g
Sodium nitrite .............................................................................0.35g
Bromthymol Blue .......................................................................0.04g
Phenol Red.................................................................................8.0mg
Glucose solution ......................................................................15.0mL
pH 6.7 ± 0.2 at 25°C
Source: This medium, without glucose solution, is available as a pre-
mixed powder from HiMedia.
Glucose Solution:
Composition per 20.0mL:
Glucose .......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 20.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute into tubes in
10.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Al-
low tubes to cool in a slanted position to form a 3-inch slant with a 1.5-
inch butt. Just before inoculation add 0.15mL sterile glucose solution
to each slant.
Use:  For the cultivation and differentiation of nonfermentative Gram-
negative bacilli.