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Semisolid Medium, Modified with Sodium Chloride and Neutral Red (BAM M30d)
[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]
Semisolid Medium, Modified
with Sodium Chloride and Neutral Red
(BAM M30d)
Peptone........................................................................................10.0g
Lactalbumin hydrolysate...............................................................5.0g
Yeast extract..................................................................................5.0g
Αgar ..............................................................................................1.8g
α-Ketoglutaric acid.......................................................................1.0g
NaCO 3 ...........................................................................................0.6g
Na-pyruvate ..................................................................................0.5g
Na 2 S 2 O 5 ........................................................................................0.5g
Hemin..........................................................................................0.01g
Neutral Red solution................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Neutral Red Solution:
Composition per 100.0mL:
Neutral Red...................................................................................0.2g
Ethanol.....................................................................................10.0mL
Preparation of Neutral Red Solution: Add 0.2g Neutral Red to
10.0mL ethanol. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Cool to 50°C. Adjust pH to 7.4. Distribute into screw-
capped tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the biochemical identification of Campylobacter spp.
with Sodium Chloride and Neutral Red
(BAM M30d)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
NaCl............................................................................................35.0gPeptone........................................................................................10.0g
Lactalbumin hydrolysate...............................................................5.0g
Yeast extract..................................................................................5.0g
Αgar ..............................................................................................1.8g
α-Ketoglutaric acid.......................................................................1.0g
NaCO 3 ...........................................................................................0.6g
Na-pyruvate ..................................................................................0.5g
Na 2 S 2 O 5 ........................................................................................0.5g
Hemin..........................................................................................0.01g
Neutral Red solution................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Neutral Red Solution:
Composition per 100.0mL:
Neutral Red...................................................................................0.2g
Ethanol.....................................................................................10.0mL
Preparation of Neutral Red Solution: Add 0.2g Neutral Red to
10.0mL ethanol. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Cool to 50°C. Adjust pH to 7.4. Distribute into screw-
capped tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the biochemical identification of Campylobacter spp.
