Semisolid Medium for Motility

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Semisolid Medium for Motility

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Biosate..........................................................................................5.0g
Polypeptone ™ ...............................................................................5.0g
NaCl..............................................................................................5.0g
Agar..............................................................................................4.0g
Myosate.........................................................................................1.5g
Triphenyltetrazolium chloride solution .....................................2.5mL
pH 6.9–7.1at 25°C
Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
Triphenyltetrazolium chloride ......................................................0.1g
Ethanol (95% solution)............................................................10.0mL
Preparation of Triphenyltetrazolium Chloride Solution :
Add triphenyltetrazolium chloride to 10.0mL of ethanol. Mix thor-
oughly.
Preparation of Medium: Add components, except triphenyltetra-
zolium chloride solution, to distilled/deionized water and bring volume
to 997.5mL. Mix thoroughly. Gently heat and bring to boiling. Add
2.5mL of triphenyltetrazolium chloride solution. Mix thoroughly. Dis-
tribute into tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the differentiation of bacteria based on motility.