Semisolid Pectin Agar

[所属分类:培养基配方] [发布时间:2021-8-13] [发布人:网站管理员2] [阅读次数:] [返回]

Semisolid Pectin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pectin..........................................................................................30.0g
Yeast extract..................................................................................5.0g
Agar..............................................................................................3.0g
Bromthymol Blue (0.1% solution) ............................................1.0mL
CaCl 2 ·2H 2 O (10% solution).......................................................0.6mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add approximately 100.0mL of distilled/
deionized water to a 2.0L flask. Place on a magnetic stirrer without
heat. Slowly add the CaCl 2 ·2H 2 O solution, Bromthymol Blue solution,
yeast extract, and pectin while stirring. Mix thoroughly to ensure uni-
form wetting of the particles. Add agar. Gently heat and bring to almost
boiling. Adjust pH to 7.3 with 1N NaOH if necessary. Do not overshoot
pH above 7.3. Distribute into tubes or flasks. Autoclave for 15 min at
15 psi pressure–121°C.
Use: For the isolation and cultivation of bacteria such as Erwinia spe-
cies and some Klebsiella species based on their ability to degrade pec-
tin.