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Sensitivity Test HiVeg Medium with Blood Serum

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Glucose ....................................................................................... 10.0g

Veal, infusion from ..................................................................... 10.0g

Plant peptone No. 3..................................................................... 10.0g

NaCl .............................................................................................. 3.0g

Na2HPO4 ....................................................................................... 2.0g

Sodium acetate.............................................................................. 1.0g

Guanine....................................................................................... 0.01g

Uracil .......................................................................................... 0.01g

Xanthine...................................................................................... 0.01g

Adenine sulfate ........................................................................... 0.01g

Sterile bovine or sheep blood ..................................................50.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed pow

der from HiMedia.

Preparation of Medium: Add components, except blood, to dis

tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile bo

vine blood or sheep blood. Mix thoroughly. Pour into sterile Petri

dishes.

Use: For antimicroibal sensitivity testing of sulfonamides and other

antimicrobics.