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Serratia Differential Medium (SD Medium)

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Serratia Differential Medium

(SD Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 102.0mL:

Solution A................................................................................92.0mL

Solution B ................................................................................10.0mL

pH 6.7 ± 0.2 at 25°C

Solution A:

Composition per 92.0mL:

Yeast extract.................................................................................. 1.0g

L-Ornithine .................................................................................... 1.0g

NaCl.............................................................................................. 0.5g

Agar .............................................................................................. 0.4g

Irgasan inhibitor.........................................................................1.0mL

Indicator solution.......................................................................1.0mL

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 92.0mL. Mix thoroughly. Adjust pH to 6.7

with 1N NaOH.

Irgasan Inhibitor:

Composition per 100.0mL:

Irgasan-DP-300 (4,2′, 4′-trichloro-

2-hydroxydiphenylether)........................................................ 0.1g

NaOH (1N solution).................................................................10.0mL

Preparation of Irgasan Inhibitor: Add irgasan to 10.0mL of

NaOH solution. Mix thoroughly. Gently heat to dissolve. Bring volume

to 100.0mL with distilled/deionized water.

Indicator Solution:

Composition per 100.0mL:

Bromthymol Blue ......................................................................... 0.2g

Phenol Red.................................................................................... 0.1g

Preparation of Indicator Solution: Add components to 50.0mL

of distilled/deionized water. Mix thoroughly for 1 hr. Bring volume to

100.0mL with distilled/deionized water.

Solution B:

Composition per 10.0mL:

L-Arabinose................................................................................... 1.0g

Preparation of Solution B: Add L-arabinose to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly.

Preparation of Medium: Combine 92.0mL of solution A with

10.0mL of solution B. Mix thoroughly. Distribute into tubes. Auto

clave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in an

upright position.

Use: For the cultivation and differentiation of Serratia species based

on the fermentation of arabinose and production of ornithine decarboxy

lase. Serratia marcescens changes the medium to purple throughout the

tube. Serratia liquefaciens changes the medium to a band of purple at

the top of the tube with a green/yellow butt. Serratia rubidaea changes

the medium to yellow throughout the tube.

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Serratia Differential Medium (SD Medium)