培养基配方

您现在的位置：网站首页 >> 培养基配方

Serum Glucose Agar (Serum Dextrose Agar) (ATCC Medium 287)

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Serum Glucose Agar

(Serum Dextrose Agar)

(ATCC Medium 287)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1060.0mL:

Agar ............................................................................................ 15.0g

Peptone ....................................................................................... 10.0g

Beef extract................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Serum-glucose solution ...........................................................60.0mL

pH 7.3 ± 0.2 at 25°C

Serum-Glucose Solution:

Composition per 60.0mL:

D-Glucose.................................................................................... 10.0g

Serum (inactivated at 56°C, 30 min) .......................................50.0mL

Preparation of Serum-Glucose Solution: Add glucose to

50.0mL of heat-inactivated serum. Horse serum or ox serum may be

used. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except serum-glucose

solution, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

10 psi pressure–115°C. Cool to 50°C. Aseptically add 60.0mL of ster

ile serum-glucose solution. Mix thoroughly. Pour into sterile Petri dish

es or distribute into sterile tubes. Allow tubes to cool in a slanted

position.

Use: For the cultivation and maintenance of Brucella species.