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Serum Glucose Agar, Farrell Modified

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1086.9mL:

Agar ............................................................................................ 15.0g

Peptone ....................................................................................... 10.0g

Beef extract................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Serum-glucose solution ...........................................................60.0mL

Bacitracin solution...................................................................12.5mL

Cycloheximide solution...........................................................10.0mL

Nystatin solution........................................................................2.0mL

Polymyxin B solution ................................................................1.0mL

Nalidixic acid solution...............................................................1.0mL

Vancomycin solution .................................................................0.4mL

pH 7.3 ± 0.2 at 25°C

Serum-Glucose Solution:

Composition per 60.0mL:

D-Glucose.................................................................................... 10.0g

Serum (inactivated at 56°C, 30 min) .......................................50.0mL

Preparation of Serum-Glucose Solution: Add glucose to

50.0mL of heat-inactivated serum. Horse serum or ox serum may be

used. Mix thoroughly. Filter sterilize.

Bacitracin Solution:

Composition per 12.5mL:

Bacitracin............................................................................... 25,000U

Preparation of Bacitracin Solution: Add Bacitracin to distilled/

deionized water and bring volume to 12.5mL. Mix thoroughly. Filter

sterilize.

Cycloheximide Solution:

Composition per 100.0mL:

Cycloheximide.............................................................................. 1.0g

Acetone......................................................................................5.0mL

Preparation of Cycloheximide Solution: Add cycloheximide to

5.0mL of acetone. Mix thoroughly. Bring volume to 100.0mL with dis

tilled/deionized water. Mix thoroughly. Filter sterilize.

Nystatin Solution:

Composition per 5.0mL:

Nystatin................................................................................ 250,000U

Preparation of Nystatin Solution: Add nystatin to distilled/de

ionized water and bring volume to 5.0mL. Mix thoroughly. Filter ster

ilize.

Polymyxin B Solution:

Composition per 2.0mL:

Polymyxin B .......................................................................... 10,000U

Preparation of Polymyxin B Solution: Add polymyxin B to dis

tilled/deionized water and bring volume to 2.0mL. Mix thoroughly. Fil

ter sterilize.

Nalidixic Acid Solution:

Composition per 2.0mL:

Nalidixic acid................................................................................ 0.1g

NaOH (0.5N solution)................................................................2.0mL

Preparation of Nalidixic Acid Solution: Add nalidixic acid to

2.0mL of NaOH solution. Mix thoroughly. Immediately before use,

add 1.0mL of this stock solution to 9.0mL of distilled/deionized water.

Mix thoroughly. Filter sterilize.

Vancomycin Solution:

Composition per 1.0mL:

Vancomycin ................................................................................ 0.05g

Preparation of Vancomycin Solution: Add vancomycin to dis

tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil

ter sterilize.

Preparation of Medium: Add components—except serum-glucose

solution, bacitracin solution, cycloheximide solution, nystatin solution,

polymyxin B solution, nalidixic acid solution, and vancomycin solu

tion—to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 10 psi

pressure–115°C. Cool to 50°C. Aseptically add 60.0mL of sterile se

rum-glucose solution, 12.5mL of sterile bacitracin solution, 10.0mL of

sterile cycloheximide solution, 2.0mL of sterile nystatin solution,

1.0mL of sterile polymyxin B solution, 1.0mL of sterile nalidixic acid

solution, and 0.4mL of sterile vancomycin solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes

to cool in a slanted position.

Use: For the selective isolation and cultivation of Brucella species.

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Serum Glucose Agar, Farrell Modified