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Serum Potato Infusion Agar

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1120.0mL:

Agar ............................................................................................ 15.0g

Peptone........................................................................................ 10.0g

Meat extract .................................................................................. 5.0g

NaCl .............................................................................................. 5.0g

Potato infusion ..............................................................................1.0L

Horse serum, heat inactivated................................................100.0mL

Glycerol ...................................................................................20.0mL

pH 6.8 ± 0.2 at 25°C

Potato Infusion:

Composition per 10.0mL:

Potatoes..................................................................................... 250.0g

Preparation of Potato Infusion: Add peeled, thinly sliced pota

toes to 1.0L of distilled/deionized water. Infuse overnight at 60°C. Fil

ter through Whatman #1 filter paper. Bring volume to 1.0L with

distilled/deionized water.

Preparation of Medium: Combine components, except horse se

rum. Mix thoroughly. Gently heat and bring to boiling. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add

100.0mL of sterile horse serum. Mix thoroughly. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the cultivation of Brucella species.