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Serum Tellurite Agar

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Pancreatic digest of casein.......................................................... 10.0g

Peptic digest of animal tissue ..................................................... 10.0g

NaCl.............................................................................................. 5.0g

Glucose ......................................................................................... 2.0g

Lamb serum .............................................................................50.0mL

Chapman tellurite solution.......................................................10.0mL

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Chapman Tellurite Solution:

Composition per 100.0mL:

K2TeO3.......................................................................................... 1.0g

Preparation of Chapman Tellurite Solution: Add K2TeO3 to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except lamb serum and

Chapman tellurite solution, to distilled/deionized water and bring vol

ume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile lamb serum and 10.0mL of sterile Chapman tel

lurite solution. Mix thoroughly. Pour into sterile Petri dishes or distrib

ute into sterile tubes.

Use: For the isolation and cultivation of Corynebacterium species,

especially in the laboratory diagnosis of diphtheria.