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Seven H11 Agar (Selective 7H11 Agar)

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Seven H11 Agar

(Selective 7H11 Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Agar ............................................................................................ 13.5g

KH2PO4......................................................................................... 1.5g

Na2HPO4....................................................................................... 1.5g

Pancreatic digest of casein............................................................ 1.0g

NaCl............................................................................................ 0.85g

Monosodium glutamate ................................................................ 0.5g

(NH4)2SO4 .................................................................................... 0.5g

Sodium citrate............................................................................... 0.4g

MgSO4·7H2O.............................................................................. 0.05g

Ferric ammonium citrate............................................................. 0.04g

CuSO4·5H2O.............................................................................. 1.0mg

Pyridoxine.................................................................................. 1.0mg

ZnSO4·7H2O .............................................................................. 1.0mg

Biotin ......................................................................................... 0.5mg

CaCl2·2H2O ............................................................................... 0.5mg

Malachite Green....................................................................... 0.25mg

Middlebrook OADC enrichment ...........................................100.0mL

Antibiotic inhibitor ..................................................................10.0mL

Glycerol .....................................................................................5.0mL

pH 6.6 ± 0.2 at 25°C

Source: This medium is available as a prepared medium from BD Di

agnostic Systems.

Middlebrook OADC Enrichment:

Composition per liter:

Bovine albumin fraction V ........................................................... 5.0g

Glucose ......................................................................................... 2.0g

NaCl ............................................................................................ 0.85g

Catalase......................................................................................3.0mg

Oleic acid .................................................................................0.06mL

Preparation of Middlebrook OADC Enrichment: Add com

ponents to distilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Filter sterilize.

Antibiotic Inhibitor:

Composition per 10.0mL:

Carbenicillin................................................................................ 0.05g

Trimethoprim lactate................................................................... 0.02g

Amphotericin B........................................................................... 0.01g

Polymyxin B ........................................................................ 200,000U

Preparation of Antibiotic Inhibitor: Add components to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add glycerol to 900.0mL of distilled/de

ionized water. Mix thoroughly. Add remaining components, except

Middlebrook OADC enrichment and antibiotic inhibitor. Mix thor

oughly. Gently heat. Do not boil. Autoclave for 10 min at 15 psi pres

sure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile

Middlebrook OADC enrichment and 10.0mL of sterile antibiotic solu

tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the isolation and cultivation of Mycobacterium species from

specimens with a mixed flora.

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Seven H11 Agar (Selective 7H11 Agar)