培养基配方

您现在的位置：网站首页 >> 培养基配方

Seven-Hour Fecal Coliform Agar (Seven-Hour FC Agar) (m-Seven-Hour Fecal Coliform Agar)

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Seven-Hour Fecal Coliform Agar

(Seven-Hour FC Agar)

(m-Seven-Hour Fecal Coliform Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Lactose ........................................................................................ 10.0g

NaCl .............................................................................................. 7.5g

D-Mannitol..................................................................................... 5.0g

Proteose peptone No. 3 ................................................................. 5.0g

Yeast extract.................................................................................. 3.0g

Bromcresol Purple ...................................................................... 0.35g

Phenol Red.................................................................................... 0.3g

Sodium lauryl sulfate .................................................................... 0.2g

Sodium deoxycholate.................................................................... 0.1g

pH 7.3 ± 0.1 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Continue boiling for 5 min. Cool to 55°–60°C. Adjust pH to

7.3 with 0.1N NaOH. Cool to 45°–50°C. Pour into sterile Petri dishes

with tight-fitting lids in 5.0mL volumes. Store at 2°–10°C.

Use: For the rapid estimation of the bacteriological quality of water

using the membrane filter method.