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Shapton Medium

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Shapton Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Peptic digest of animal tissue........................................................ 5.0g

Beef extract ................................................................................... 3.0g

Casein enzymic hydrolysate ......................................................... 2.5g

Glucosec........................................................................................ 1.0g

Yeast extract.................................................................................. 1.0g

Bromo Cresol Purple ................................................................ 0.025g

pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the cultivation of aciduric and thermophilic flat sour spore

formers. For the enumeration of spores of Bacillus stearothermophilus

which cause flat sour spoilage in canned foods with pH more than 4.5.