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Sheep Blood Agar (BAM M135)

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Sheep Blood Agar

(BAM M135)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone ......................................................................... 15.0g

Agar ............................................................................................ 12.0g

Liver digest ................................................................................... 2.5g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 5.0g

Sheep blood, defibrinated ........................................................50.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 950.0mL. Mix thorough

ly. Heat with frequent agitation and boil for 1 min to completely dis

solve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

46°C. Aseptically add 50.0mL of sterile, defibrinated sheep blood. Mix

thoroughly and pour into sterile Petri dishes.

Use: For the isolation, cultivation, and detection of hemolytic activity

of streptococci and other fastidious microorganisms.