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Sheep Blood Agar

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Sheep Blood Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone...................................................................................... 14.0g

Agar ............................................................................................ 12.0g

NaCl .............................................................................................. 5.0g

Peptone, neutralized...................................................................... 4.5g

Yeast extract.................................................................................. 4.5g

Sheep blood, defibrinated ........................................................70.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Preparation of Medium: Add components, except sheep blood, to

distilled/deionized water and bring volume to 930.0mL. Mix thorough

ly. Heat with frequent agitation and boil for 1 min to completely dis

solve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Aseptically add 70.0mL of sterile, defibrinated sheep blood. Mix thor

oughly and pour into sterile Petri dishes.

Use: For the isolation, cultivation, and detection of hemolytic activity

of streptococci and other fastidious microorganisms. Specifically for

mulated to give maximum recovery and improved hemolytic reactions

with sheep blood.