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培养基配方

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Siderophore Mineral Medium

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4......................................................................................... 8.2g

NaOH ............................................................................................ 1.6g

NH4Cl ........................................................................................... 1.0g

KCl................................................................................................ 0.5g

CaSO4·2H2O ..............................................................................0.5mg

CuSO4·5H2O ..............................................................................0.5mg

FeCl3·6H2O ................................................................................0.5mg

ZnSO4·7H2O ..............................................................................0.5mg

Deferrioxamine B solution.......................................................10.0mL

MgSO4·7H2O solution .............................................................10.0mL

Wolfe’s vitamin solution ............................................................5.0mL

Deferrioxamine B Solution:

Composition per 10.0mL:

Deferrioxamine B ......................................................................... 1.0g

Preparation of Deferrioxamine B Solution: Add deferrioxam

ine B to distilled/deionized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

MgSO4·7H2O Solution:

Composition per 10.0mL:

MgSO4·7H2O ................................................................................ 0.5g

Preparation of MgSO4·7H2O Solution: Add MgSO4·7H2O to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Wolfe’s Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ........................................................................... 0.01g

Thiamine·HCl ............................................................................ 5.0mg

Riboflavin .................................................................................. 5.0mg

Nicotinic acid............................................................................. 5.0mg

Calcium pantothenate ................................................................ 5.0mg

p-Aminobenzoic acid................................................................. 5.0mg

Thioctic acid .............................................................................. 5.0mg

Biotin ......................................................................................... 2.0mg

Folic acid ................................................................................... 2.0mg

Cyanocobalamin ........................................................................ 0.1mg

Preparation of Wolfe’s Vitamin Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Fil

ter sterilize.

Preparation of Medium: Add components—except deferrioxam

ine B solution, MgSO4·7H2O solution, and Wolfe’s vitamin solution—

to distilled/deionized water and bring volume to 975.0mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL of sterile

deferrioxamine B solution, 10.0mL of sterile MgSO4·7H2O solution,

and 5.0mL of sterile Wolfe’s vitamin solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of ATCC strain 49538.

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Siderophore Mineral Medium