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Sierra Medium

[所属分类:培养基配方] [发布时间:2021-8-16] [发布人:网站管理员2] [阅读次数:] [返回]

Sierra Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Peptone ....................................................................................... 10.0g

NaCl.............................................................................................. 5.0g

CaCl2·H2O .................................................................................... 0.1g

Tween™ 80..............................................................................10.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, except Tween™ 80, to

distilled/deionized water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Separately autoclave Tween™ 80 for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add

10.0mL of sterile Tween™ 80. Mix thoroughly. Pour into sterile Petri

dishes.

Use: For the differentiation of bacteria based on lipase activity. Bacte

ria with lipase activity form colonies surrounded by a white precipitate.