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Simonsiella Agar (LMG Medium 31)

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Simonsiella Agar

(LMG Medium 31)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptone...................................................................................... 17.0g

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 4.0g

Soy peptone .................................................................................. 3.0g

K2HPO4......................................................................................... 2.5g

Bovine serum.........................................................................100.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except bovine serum,

to distilled/deionized water and bring volume to 900.0mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL sterile

bovine serum. Mix thoroughly. Pour into sterile Petri dishes or distrib

ute into sterile tubes.

Use: For the cultivation of Simonsiella spp.