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Simonsiella Agar

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Simonsiella Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 17.0g

Agar ............................................................................................ 15.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 4.0g

Papaic digest of soybean meal...................................................... 3.0g

K2HPO4......................................................................................... 2.5g

Horse serum...........................................................................100.0mL

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°–55°C. Aseptically add 100.0mL of sterile

horse serum warmed to 50°–55°C. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Simonsiella muelleri and

Simonsiella steedae.