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Single-Layer Agar

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Single-Layer Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Tributyrin substrate..................................................................... 50.0g

Basal medium ...............................................................................1.0L

pH 6.8 ± 0.2 at 25°C

Basal Medium:

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of gelatin........................................................... 5.0g

Beef extract................................................................................... 3.0g

Preparation of Basal Medium: Add components to 1.0L of dis

tilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 50°C.

Tributyrin Substrate:

Composition:

Tributyrin substrate..................................................................... 50.0g

Preparation of Tributyrin Substrate: Remove free fatty acids in

the tributyrin substrate by dissolving 50.0g in 500.0mL of petroleum

ether. Pass the solution through an activated alumina column. Remove

the petroleum ether by evaporation on a steam table under 100% N2.

Autoclave for 30 min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine 1.0L of sterile basal

medium with 50.0g of sterile tributyrin substrate in a warm, sterile

blender container. Blend for 1 min until homogenized. Rapidly pour

into sterile Petri dishes in 7.0mL volumes. Dry the surface of the plates

by partially opening the lids in a laminar flow hood for 15 min.

Use: For the isolation, cultivation, and identification of lipolytic micro

organisms from food.