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Single-Layer Agar

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Single-Layer Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

Fat substrate................................................................................ 50.0g

Basal medium ...............................................................................1.0L

pH 6.8 ± 0.2 at 25°C

Basal Medium:

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of gelatin........................................................... 5.0g

Beef extract................................................................................... 3.0g

Victoria Blue B solution ........................................................200.0mL

Preparation of Basal Medium: Add agar to 800.0mL of distilled/

deionized water. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°C. Aseptically add 200.0mL of Victoria Blue B solution. Mix

thoroughly.

Victoria Blue B Solution:

Composition per 200.0mL:

Victoria Blue B ........................................................................... 0.12g

Preparation of Victoria Blue B Solution: Add the Victoria Blue

B to 200.0mL of distilled/deionized water. Mix thoroughly. Filter ster

ilize. Warm to 50°C.

Fat Substrate:

Composition:

Fat substrate................................................................................ 50.0g

Preparation of Fat Substrate: Corn oil, soybean oil, any cooking

oil, lard, tallow, or triglycerides that do not contain antioxidants or oth

er inhibitory substances may be used. Remove free fatty acids in the fat

substrate by dissolving 50.0g of fat substrate in 500.0mL of petroleum

ether. Pass the solution through an activated alumina column. Remove

the petroleum ether by evaporation on a steam table under 100% N2.

Autoclave for 30 min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine 1.0L of sterile basal

medium with 50.0g of sterile fat substrate in a warm, sterile blender

container. Blend for 1 min until homogenized. Rapidly pour into sterile

Petri dishes in 7.0mL volumes. Dry the surface of the plates by partial

ly opening the lids in a laminar flow hood for 15 min.

Use: For the isolation, cultivation, and identification of lipolytic micro

organisms from food.