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Singulosphaera Medium (DSMZ Medium 1144)

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Singulosphaera Medium

(DSMZ Medium 1144)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar-agar .................................................................................... 18.0g

N-acetylglucosamine ................................................................... 1.0g

KH2PO4......................................................................................... 0.1g

Peptone ......................................................................................... 0.1g

Yeast extract ................................................................................. 0.1g

Hutner’s basal salts solution ....................................................20.0mL

Ampicillin soluiton ..................................................................10.0mL

pH 5.8 ± 0.2 at 25°C

Hutner’s Basal Salts Solution:

Composition per liter:

MgSO4·7H2O .............................................................................. 29.7g

Nitrilotriacetic acid ..................................................................... 10.0g

CaCl2·2H2O............................................................................... 3.335g

FeSO4·7H2O.............................................................................99.0mg

(NH4)6Mo7O24·4H2O ...............................................................9.25mg

"Metals 44" ..............................................................................50.0mL

“Metals 44”:

Composition per 100.0mL:

ZnSO4·7H2O ............................................................................. 1.095g

FeSO4·7H2O.................................................................................. 0.5g

Sodium EDTA............................................................................. 0.25g

MnSO4·H2O.............................................................................. 0.154g

CuSO4·5H2O ............................................................................39.2mg

Co(NO3)2·6H2O .......................................................................24.8mg

Na2B4O7·10H2O.......................................................................17.7mg

Preparation of “Metals 44”: Add sodium EDTA to distilled/de

ionized water and bring volume to 90.0mL. Mix thoroughly. Add a few

drops of concentrated H2SO4 to retard precipitation of heavy metal

ions. Add remaining components. Mix thoroughly. Bring volume to

100.0mL with distilled/deionized water.

Preparation of Hutner’s Basal Salts Solution: Add nitrilotria

cetic acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5

with KOH. Add remaining components. Add distilled/deionized water

to 1.0L. Adjust pH to 6.8. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C.

Ampicillin Solution:

Composition per 10.0mL:

Na-ampicillin ............................................................................... 0.2g

Preparation of Ampicillin Solution: Add Na-ampicillin to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except Hutner’s basal

salts solution and ampicillin solution, to distilled/deionized water and

bring volume to 970.0L. Mix thoroughly. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.

Aseptically add Hutner’s basal salts solution and ampicillin solution.

Mix thoroughly. Adjust pH to 5.8. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation of Singulosphaera spp.

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Singulosphaera Medium (DSMZ Medium 1144)