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Skim Milk Agar

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Skim Milk Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Agar ............................................................................................ 15.0g

Pancretic digest of casein.............................................................. 5.0g

Yeast extract.................................................................................. 2.5g

Glucose ......................................................................................... 1.0g

Skim milk solution.................................................................100.0mL

pH 7.0 ± 0.1 at 25°C

Preparation of Skim Milk Solution: Add skim milk solids to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Add components, except skim milk solu

tion, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Distribute into tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 100.0mL of cooled, sterile skim milk solution. Mix

thoroughly. Pour into sterile Petri dishes or aseptically distribute into

sterile tubes.

Use: For the cultivation and differentiation of bacteria based on prote

olytic activity.