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Skim Milk Agar, Half Strength

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................500.0mL

Solution B ..............................................................................500.0mL

Solution A:

Composition per 500.0mL:

Skim milk.................................................................................... 25.0g

Preparation of Solution A: Add 25.0g of skim milk to distilled/de

ionized water and bring volume to 500.0mL. Mix thoroughly. Adjust

pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–

55°C.

Solution B:

Composition per 500.0mL:

Agar ............................................................................................ 20.0g

Preparation of Solution B: Add 20.0g of agar to distilled/deion

ized water and bring volume to 500.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 50°–55°C.

Preparation of Medium: Aseptically mix 500.0mL of solution A

with 500.0mL of solution B. Pour into sterile Petri dishes or leave in

tubes.

Use: For the cultivation and maintenance of Pilimelia anulata.