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Skim Milk Glucose Agar (DSMZ Medium 810)

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Skim Milk Glucose Agar

(DSMZ Medium 810)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................500.0mL

Solution B ..............................................................................500.0mL

pH 6.8 ± 0.2 at 25°C

Solution A:

Composition per 500.0mL:

Skim milk.................................................................................... 40.0g

Preparation of Solution A: Add skim milk to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Solution B:

Composition per 500.0mL:

Glucose ....................................................................................... 20.0g

Agar ............................................................................................ 20.0g

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically mix 500.0mL sterile solution

A and 500.0mL sterile solution B. Mix thoroughly. Adjust pH to 6.8.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Trichophyton spp.