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SL Medium (DSMZ Medium 959)

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SL Medium

(DSMZ Medium 959)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 15.0g

PIPES............................................................................................ 3.4g

Na-acetate·3H2O......................................................................... 2.72g

Yeast extract.................................................................................. 2.0g

Trypticase™.................................................................................. 2.0g

NH4Cl ........................................................................................... 1.0g

MgCl2·6H2O ................................................................................. 0.5g

K2HPO4....................................................................................... 0.35g

KH2PO4....................................................................................... 0.35g

KCl................................................................................................ 0.2g

CaCl2·2H2O .................................................................................. 0.1g

Resazurin ................................................................................... 0.5mg

Maltose solution.......................................................................50.0mL

Na2S·9H2O solution.................................................................10.0mL

pH 7.5 ± 0.2 at 25°C

Maltose Solution:

Composition per 100.0mL:

Maltose ......................................................................................... 3.5g

Preparation of Maltose Solution: Add maltose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Sparge with

N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Store anaerobically.

Na2S·9H2O Solution:

Composition per 50.0mL:

Na2S·9H2O.................................................................................... 5.0g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 50.0mL. Sparge with N2.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Store an

aerobically.

Preparation of Medium: Add components, except maltose solu

tion and Na2S·9H2 solution, to distilled/deionized water and bring vol

ume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.

Sparge with N2. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 25°C. Aseptically add 10.0mL sterile Na2S·9H2O

solution and 50.0mL sterile maltose solution. Mix thoroughly. Aspti

cally and anaerobically distribute into sterile tubes or flasks under N2.

The final pH should be 7.5.

Use: For the cultivation of Petrotoga olearia and Petrotoga sibirica.