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SL Medium

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SL Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Pancreatic digest of casein.......................................................... 10.0g

Glucose ....................................................................................... 10.0g

KH2PO4......................................................................................... 6.0g

Arabinose...................................................................................... 5.0g

Sucrose.......................................................................................... 5.0g

Yeast extract.................................................................................. 5.0g

Sodium acetate·3H2O.................................................................... 2.5g

Diammonium citrate ..................................................................... 2.0g

Tween™ 80................................................................................... 1.0g

MgSO4·7H2O.............................................................................. 0.58g

MnSO4·4H2O.............................................................................. 0.28g

pH 5.4 ± 0.2 at 25°C

Preparation of Medium: Add agar to 500.0mL of distilled/deion

ized water. Gently heat and bring to boiling. In a separate flask, add the

remaining components, except sodium acetate, to distilled/deionized

water and bring volume to 300.0mL. Mix thoroughly. Combine the two

solutions. In a separate flask, add sodium acetate to distilled/deionized

water and bring volume to100.0mL. Mix thoroughly. Adjust pH to 5.4

with glacial acetic acid. Add this solution to the agar solution. Mix

thoroughly. Adjust pH to 5.4 with additional glacial acetic acid. Bring

volume to 1.0L with distilled/deionized water. Do not autoclave. Asep

tically distribute into sterile screw-capped tubes or flasks.

Use: For the selective isolation and cultivation of Lactobacillus spe

cies.