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培养基配方
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Slad Medium
[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]
Slad Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Solution B ..............................................................................900.0mL
Solution A ..............................................................................100.0mL
Solution A:
Composition per 100.0mL:
Raffinose..................................................................................... 20.0g
KH2PO4......................................................................................... 1.0g
MgSO4·7H2O ................................................................................ 0.5g
NaCl .............................................................................................. 0.1g
CaCl2·2H2O................................................................................... 0.1g
Ammonium sulfate ....................................................................7.0mg
Inositol .......................................................................................2.0mg
KI ...............................................................................................1.0mg
H3BO3 ........................................................................................0.5mg
ZnSO4·7H2O ..............................................................................0.4mg
MnSO4·4H2O .............................................................................0.4mg
Thiamine·HCl ............................................................................0.4mg
Pyroxidine·HCl ..........................................................................0.4mg
Niacin.........................................................................................0.4mg
Calcium pantothenate ................................................................0.4mg
p-Aminobenzoic acid.................................................................0.2mg
Riboflavin ..................................................................................0.2mg
FeCl3 ..........................................................................................0.2mg
Na2MoO4·4H2O .........................................................................0.2mg
CuSO4·5H2O ............................................................................0.04mg
Folic acid.....................................................................................2.0μg
Biotin ..........................................................................................2.0μg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Warm to 50°–55°C.
Solution B:
Composition per 900.0mL:
Agar ............................................................................................ 40.0g
Preparation of Solution B: Add agar to distilled/deionized water
and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically combine 100.0mL of sterile
solution A and 900.0mL of sterile solution B. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Saccharomyces cerevisiae.