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Slanetz and Bartley, HiVeg (Slanetz and Bartley HiVeg Medium)

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

Slanetz and Bartley, HiVeg

(Slanetz and Bartley HiVeg Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate No. 1............................................................... 20.0g

Agar ............................................................................................ 15.0g

Yeast extract.................................................................................. 5.0g

Na2HPO4....................................................................................... 4.0g

Glucose ......................................................................................... 2.0g

NaN3 ............................................................................................. 0.4g

2,3,5-Triphenyltetrazolium chloride ............................................. 0.1g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Caution: Sodium azide is toxic. Azides also react with metals and

disposal must be highly diluted.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling to dissolve the medium completely. Excessive heating

should be avoided. Do not autoclave. Pour into sterile Petri dishes or

leave in tubes.

Use: For the detection and enumeration of fecal streptococci by the

membrane filter technique.