培养基配方

您现在的位置：网站首页 >> 培养基配方

SM Medium

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SM Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4 ....................................................................................... 4.5g

KH2PO4......................................................................................... 1.5g

NH4Cl ........................................................................................... 0.3g

MgSO4·7H2O ................................................................................ 0.1g

Na2S2O3 solution....................................................................100.0mL

Trace metals solution .................................................................5.0mL

pH 7.5 ± 0.2 at 25°C

Na2S2O3 Solution

Composition per 100.0mL:

Na2S2O3 solution......................................................................... 10.0g

Preparation of Na2S2O3 Solution: Add 10.0g of Na2S2O3 to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Filter sterilize.

Trace Metals Solution:

Composition per liter:

Ethylenediaminetetraacetic acid ................................................. 50.0g

ZnSO4·7H2O ............................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2·4H2O................................................................................ 5.06g

FeSO4·7H2O................................................................................ 4.99g

CoCl2·6H2O ................................................................................ 1.61g

CuSO4·5H2O ............................................................................... 1.57g

(NH4)6Mo7O24·H2O .................................................................... 1.10g

Preparation of Trace Metals Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 6.0 using KOH. Filter sterilize.

Preparation of Medium: Add components, except Na2S2O3 solu

tion and trace metals solution, to distilled/deionized water and bring

volume to 895.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi

pressure–121°C. Aseptically add 100.0mL of sterile Na2S2O3 solution

and 5.0mL of sterile trace metals solution. Mix thoroughly. Aseptically

distribute into sterile tubes or flasks.

Use: For the cultivation of Thiobacillus thioparus.