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SM Selective Medium

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SM Selective Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Mannitol........................................................................................ 2.5g

L-Glutamic acid............................................................................. 1.0g

MgSO4·7H2O.............................................................................. 0.16g

Triphenyltetrazolium·chloride solution ...................................10.0mL

Antibiotic solution ...................................................................10.0mL

KH2PO4 (0.2mM solution).........................................................1.0mL

Metals solution.........................................................................0.05mL

pH 7.2 ± 0.2 at 25°C

Triphenyltetrazolium Chloride Solution:

Composition per 10.0mL:

2,3,5-Triphenyltetrazolium chloride ............................................. 0.1g

Ethanol (95% solution)............................................................10.0mL

Preparation of Triphenyltetrazolium Chloride Solution: Add

2,3,5-triphenyltetrazolium chloride to 10.0mL of ethanol. Mix thor

oughly.

Antibiotic Solution:

Composition per 10.0mL:

Bacitracin.................................................................................... 0.05g

Cycloheximide............................................................................ 0.05g

Tyrothricin .................................................................................. 0.02g

Captan......................................................................................... 0.01g

Vancomycin ................................................................................ 0.01g

Chloromycetin ............................................................................5.0μg

Penicillin G .................................................................................1.0μg

Preparation of Antibiotic Solution: Add components to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Metals Solution:

Composition per 100.0mL:

ZnSO4·7H2O ................................................................................. 1.1g

MnSO4·H2O................................................................................ 0.62g

Fe(NH4)2(SO4)2·6H2O ................................................................ 0.18g

CuSO4·5H2O............................................................................. 0.029g

CaSO4·5H2O ............................................................................. 0.029g

H3PO3 ....................................................................................... 0.011g

KI ........................................................................................... 0.013mg

Preparation of Metals Solution: Add components to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, except antibiotic solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Adjust pH to 7.2 with

KOH. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add sterile antibiotic solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Pseudomonas solanacearum.

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SM Selective Medium