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SMB Medium (DSMZ Medium 1185)

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SMB Medium

(DSMZ Medium 1185)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ........................................................................................... 50.0g

Tryptone ....................................................................................... 5.0g

Na2HPO4 ...................................................................................... 3.0g

KH2PO4 ........................................................................................ 2.0g

Yeast extract ................................................................................. 2.0g

NH4NO3 ........................................................................................ 1.0g

MgSO4·7H2O ................................................................................ 0.2g

Na2CO3 ......................................................................................... 0.1g

CaCl2·2H2O................................................................................. 0.01g

Manganese sulfate solution........................................................2.0mL

Iron sulfate solution ...................................................................1.0mL

pH 6.8–7.1 at 25°C

Manganese Sulfate Solution:

Composition per 10.0mL:

MnSO4·2H2O ................................................................................ 0.1g

Preparation of Manganese Sulfate Solution: Add MnSO4·2H2O

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Iron Sulfate Solution:

Composition per 10.0mL:

FeSO4·7H2O.................................................................................. 0.1g

Preparation of Iron Sulfate Solution: Add FeSO4·7H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8–7.1.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Salinicola socius.