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SMC Medium

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SMC Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sorbitol........................................................................................ 70.0g

Pancreatic digest of casein.......................................................... 17.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

Yeast extract.................................................................................. 3.0g

Beef heart, solids from infusion.................................................... 2.0g

Horse serum ...........................................................................200.0mL

Yeast extract solution .............................................................100.0mL

Phenol Red solution .................................................................20.0mL

Sucrose solution.......................................................................20.0mL

L-Arginine·HCl solution...........................................................10.0mL

Fructose solution........................................................................2.0mL

Glucose solution ........................................................................2.0mL

pH 7.5 ± 0.2 at 25°C

Yeast Extract Solution:

Composition per 100.0mL:

Yeast extract................................................................................ 25.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Phenol Red Solution:

Composition per 100.0mL:

Phenol Red.................................................................................. 0.01g

Preparation of Phenol Red Solution: Add Phenol Red to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Sucrose Solution:

Composition per 20.0mL:

Sucrose........................................................................................ 10.0g

Preparation of Sucrose Solution: Add sucrose to distilled/deion

ized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

L-Arginine·HCl Solution:

Composition per 10.0mL:

L-Arginine·HCl.............................................................................. 4.2g

Preparation of L-Arginine·HCl Solution: Add L-arginine·HCl to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Fructose Solution:

Composition per 10.0mL:

Fructose......................................................................................... 5.0g

Preparation of Fructose Solution: Add fructose to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Glucose Solution:

Composition per 10.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Add components—except horse serum,

yeast extract solution, Phenol Red solution, sucrose solution, L

arginine·HCl solution, fructose solution, and glucose solution—to dis

tilled/deionized water and bring volume to 646.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add 200.0mL of sterile

horse serum, 100.0mL of sterile yeast extract solution, 20.0mL of ster

ile Phenol Red solution, 20.0mL of sterile sucrose solution, 10.0mL of

sterile L-arginine·HCl solution, 2.0mL of sterile fructose solution, and

2.0mL of sterile glucose solution. Mix thoroughly. Aseptically distrib

ute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Spiroplasma citri.

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SMC Medium