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SMC, Modified

[所属分类:培养基配方] [发布时间:2021-8-17] [发布人:网站管理员2] [阅读次数:] [返回]

SMC, Modified

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sorbitol ....................................................................................... 70.0g

Pancreatic digest of casein.......................................................... 17.0g

NaCl.............................................................................................. 5.0g

Beef extract................................................................................... 3.0g

Yeast extract.................................................................................. 3.0g

Beef heart, solids from infusion.................................................... 2.0g

Solution 1...............................................................................100.0mL

Solution 3.................................................................................20.0mL

Solution 2.................................................................................10.0mL

NaOH (1N solution)...................................................................6.0mL

pH 7.7–7.8 at 25°C

Solution 1:

Composition per 100.0mL:

Sucrose........................................................................................ 10.0g

Yeast extract.................................................................................. 2.0g

Fructose......................................................................................... 1.0g

Glucose ......................................................................................... 1.0g

Phenol Red.................................................................................. 0.02g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution 2:

Composition per 10.0mL:

Bovine serum albumin, fraction V................................................ 0.1g

Preparation of Solution 2: Add bovine serum albumin to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Solution 3:

Composition per 20.0mL:

Horse serum .............................................................................20.0mL

Preparation of Solution 3: Inactivate horse serum at 56°C for 30

min. Filter sterilize.

Preparation of Medium: Add components—except solution 1, so

lution 2, and solution 3—to distilled/deionized water and bring volume

to 870.0mL. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 100.0mL of sterile solution 1, 20.0mL of

sterile solution 3, and 10.0mL of sterile solution 2. Mix thoroughly.

Adjust pH to 7.7–7.8. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Spiroplasma citri.